UPDATED 10/31/2011: Made a few enhancements based on feedback below. This involved fixing her cutie mark, stiffening her hooves, fixing the right hind leg, adding an overlay over her horn, and improving the blink animation.
The source file has been updated below, but the original source and gif can still be downloaded here:[link][link]
My most ambitious test of Synfig Studio yet, featuring Twilight Sparkle in her regular walking animation.
It took a while to do only because I was still getting used to using this program for something more complicated than stick figures, but once I got the ball rolling it was fairly easy-going. [although it still needs more polish, of course] ^^
All the animation was done using only 5 waypoints (6, if you count the blink), with the hair and cutie mark bobbing being handled by Warp Distort layers. The additional 5 waypoints needed to include the blink animation were added by duplicating the original 5 waypoints -- note that the Time Loop layer is only there to create the Youtube video rendering, but is not needed for the regular GIF render.
Also, since these characters aren't mine, the source is free for anyone in the community to use if they want...
Synfig Source: [link]
Original Inkscape vector: [link]
Synfig Studio: [link]
MLP:FiM @ Hasbro